The influence of western corn rootworm – Diabrotica Virgifera

نویسندگان

  • Aylin Gazdagli
  • Gulruh Albayrak
چکیده

he forage legume cicera chickling (Lathyrus cicera (L.) D.C.) disributed in Mediterranean region is gaining importance in terms f economy and agriculture in Turkey. However, full potential of he legume has to be realized yet due to the presence of neuotoxin, -N-oxalyl-l-a, -diaminopropionoc acid (ODAP) causing athyrism. This study aimed to develop efficient micropropagation ystem using longitudinally sliced cotyledon node explants for use n Agrobacterium mediated genetic transformation in the future. n this study, instead of the agar as a gelling agent alternative elling agent isubgol was used. The results showed that the maxium number of shoots per explant was achieved on MS medium olidified with 10g/l isubgol gelled medium containing 0.60mg/l AP–0.2mg/l NAA. Shoots were rooted by pulse treatment with 0mg/l IBA for 5min followed by culture on 10g/l isubgol gel solidfiedMSmedium. The results showed80% rooting in treated shoots. he rooted plantlets were transferred to pots containing sand and rganic matter in pots and acclimatised.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genome Sequence of Diabrotica virgifera virgifera virus 2, a Novel Small RNA Virus of the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte

The genome of a novel small RNA virus, tentatively named Diabrotica virgifera virgifera virus 2 (DvvV2), was identified in the western corn rootworm, Diabrotica virgifera virgifera LeConte, through transcriptome sequencing and confirmed by reverse transcription-PCR. Here, we report the near-complete nucleotide sequence and the genome organization of DvvV2.

متن کامل

RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera.

The western corn rootworm (WCR), Diabrotica virgifera virgifera, is the most important pest of corn in the US Corn Belt. Economic estimates indicate that costs of control and yield loss associated with WCR damage exceed $US 1 billion annually. Historically, corn rootworm management has been extremely difficult because of its ability to evolve resistance to both chemical insecticides and cultura...

متن کامل

Genome Sequence of the First Coleopteran Iflavirus Isolated from Western Corn Rootworm, Diabrotica virgifera virgifera LeConte

The genome sequence of a novel iflavirus was identified from the transcriptome of the western corn rootworm, Diabrotica virgifera virgifera The RNA sequence consists of 9,823 nucleotides (nt) with a 3' polyadenylated tail, containing a single open reading frame that encodes a 3,028-amino-acid polyprotein.

متن کامل

RECENT RESEARCH ON THE WESTERN CORN ROOTWORM Western corn rootworm ( Diabrotica virgifera virgifera LeConte) population dynamics

1 The western corn rootworm Diabrotica virgifera virgifera LeConte is a major insect pest of field maize, Zea mays L. Larvae can cause substantial injury by feeding on maize roots. Larval feeding may destroy individual roots or root nodes, and reduce plant growth, stability, and yield. Costs associated with managing corn rootworms in continuous maize are annually one of the largest expenditures...

متن کامل

Entomopathogenic fungi in cornfields and their potential to manage larval western corn rootworm Diabrotica virgifera virgifera.

Entomopathogenic ascomycete fungi are ubiquitous in soil and on phylloplanes, and are important natural enemies of many soil-borne arthropods including larval western corn rootworm, Diabrotica virgifera virgifera, which is a major pest of corn. We measured the prevalence of Beauveria bassiana and Metarhizium anisopliae sensu lato in ten cornfields in Iowa, USA by baiting with larval insects. B....

متن کامل

Genome Sequence of a Novel Positive-Sense, Single-Stranded RNA Virus Isolated from Western Corn Rootworm, Diabrotica virgifera virgifera LeConte

The genome sequence of a novel small RNA virus was assembled from the transcriptome of the western corn rootworm, Diabrotica virgifera virgifera The assembled genome has 13,182 nucleotides with a 3' polyadenylated tail. Two open reading frames are predicted to encode polyproteins of 2,838 and 1,073 amino acids.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2012