The influence of western corn rootworm – Diabrotica Virgifera
نویسندگان
چکیده
he forage legume cicera chickling (Lathyrus cicera (L.) D.C.) disributed in Mediterranean region is gaining importance in terms f economy and agriculture in Turkey. However, full potential of he legume has to be realized yet due to the presence of neuotoxin, -N-oxalyl-l-a, -diaminopropionoc acid (ODAP) causing athyrism. This study aimed to develop efficient micropropagation ystem using longitudinally sliced cotyledon node explants for use n Agrobacterium mediated genetic transformation in the future. n this study, instead of the agar as a gelling agent alternative elling agent isubgol was used. The results showed that the maxium number of shoots per explant was achieved on MS medium olidified with 10g/l isubgol gelled medium containing 0.60mg/l AP–0.2mg/l NAA. Shoots were rooted by pulse treatment with 0mg/l IBA for 5min followed by culture on 10g/l isubgol gel solidfiedMSmedium. The results showed80% rooting in treated shoots. he rooted plantlets were transferred to pots containing sand and rganic matter in pots and acclimatised.
منابع مشابه
Genome Sequence of Diabrotica virgifera virgifera virus 2, a Novel Small RNA Virus of the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte
The genome of a novel small RNA virus, tentatively named Diabrotica virgifera virgifera virus 2 (DvvV2), was identified in the western corn rootworm, Diabrotica virgifera virgifera LeConte, through transcriptome sequencing and confirmed by reverse transcription-PCR. Here, we report the near-complete nucleotide sequence and the genome organization of DvvV2.
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